1. 960 CSS Framework
The 960 Grid System is an effort to streamline web development workflow by providing commonly used dimensions, based on a width of 960 pixels. There are two variants: 12 and 16 columns, which can be used separately or in tandem. The 960.css file itself is only 3.6 KB compressed.
2. Blueprint CSS Framework
Blueprint is a CSS framework, which aims to cut down on your CSS development time. It gives you a solid CSS foundation to build your project on top of, with an easy-to-use grid, sensible typography, nice-looking forms and even a stylesheet for printing. One of its main feature is performing a mass reset of browser default styles, offering a methodology to use for customizable layout grids and most important is that all elements are override-able.
3. The jQuery UI CSS Framework
jQuery UI includes a robust CSS Framework designed for building custom jQuery widgets. The framework includes classes that cover a wide array of common user interface needs, and can be manipulated using jQuery UI ThemeRoller. By building your UI components using the jQuery UI CSS Framework, you will be adopting shared markup conventions and allowing for ease of code integration across the plugin community at large.
4. YAML
Yaml (Yet Another Multicolumn Layout) focused on flexible layouts and offers column and grid based design elements for flexible, elastic or fixed layouts. You will need a license to use it.
YAML comes with a a very nice Ajax builder, a handy tool for visual development of YAML-based CSS layouts which allows you to put the containers of the layout visually together via drag-n-drop. The valid HTML- and CSS-code is generated automatically on the fly.
5. Yahoo YUI Grids CSS
The foundational YUI Grids CSS offers four preset page widths, six preset templates, and the ability to stack and nest subdivided regions of two, three, or four columns. The 4kb file provides over 1000 page layout combinations. Features include: Self-clearing footer, customization of width for fixed-width layouts, Supports fluid-width (100%) layouts and more.
The YUI Grids Builder — a simple interface for Grids customization.